Graham test safe & clean REAL: increased effectiveness in the detection of oxyide

Graham test safe and clean from REAL laboratory is the name of the first test on the market that we can purchase ready for use in our work environment.

Are test carriers that we can use in the laboratory, and they have been specially prepared for the detection of Enterobius vermicularis, a small parasitic nematode of humans popularly known as “oxyide”. A very common parasite that causes an intestinal disease known as oxyuriasis or enterobiasis. What is commonly referred to as the generic name of worms.
Although the infestation of this parasite is not serious, they are usually very annoying. Thus, the most common symptoms are severe pain and itching around the anus, especially during night rest, also assuming sleep disturbance and weight loss.

The itching that causes the presence of this parasite favors the reinfesstation of it. The eggs of the nail are introduced under the nails with the scratch, contaminating the hands and facilitating their intake by the person affected by this parasite. They can also spread through the air and be inhaled by people. Eggs ingested by hosts hatch in the duodenum and mature in the large intestine, where they are housed.

 

Real laboratory Graham test safe and clean employment

The laboratory is usually diagnosed with the presence of oxyides by recovering eggs from the perianal area of the affected. This is usually done by using the Graham Test and its subsequent microscopic identification. For samples to be useful, they must be collected for 3 consecutive days to be representative. But in all of this, what is the Graham Safe & Clean Test?

It is a system composed of three glass slides with adhesive tape, which are ready for use. All of them are included in a plastic box that protects them during transport from the lab to their final destination. It also includes a flexible and soft plastic material (cap) device that attaches to one end of the slide for easy sampling.

The main advantages of the Graham Safe & Clean Test are as follows:
• This is a clean and safe system.
• Simplifies sampling.
• Avoid direct contact with the glass.
• Protects against possible contagions so the reliability of the results is very high.
• Marked with the European Union’s IVD quality seal.

The use of this type of carrier is very simple and just follow the following steps:
1. The first thing is to place the cap on the unlabeled end.
2. Remove the adhesive tape from the glass and bring it to the glass cap.
3. Place the adhesive tape over the cap.
4. Put the cap with the tape in contact with the perianal area.
5. Return the adhesive tape to its initial position, preventing bubble or crease formation in this process.
6. Remove the cap from the glass and throw it away. Store the glass in its box.

 

Graham test - DURVIZ

Graham test safe and clean de REAL laboratory

 

This product is available in several different formats: on the one hand, there is the possibility to purchase it in packs of 90 Tests (90 envelopes with 3 holders), but also in the following packages: 90 Tests (90 rigid plastic boxes with 3 holders); and 90 Tests (90 rigid plastic boxes with 3 more Safe & Clean holders). At Durviz we are specialists in providing you with the most effective tools on the market, so we constantly update our catalog of products and services.

So, if you want your lab to have the most effective tools on the market, do not hesitate and visit our website where we constantly share information about the news that we are incorporating. All of them come from the most prestigious manufacturers around the world, which have endorsements and certifications from the most important bodies internationally.

New REAL Microbiome vaginal DNA kit

REAL has developed a complete system for the study of VAGINAL MICROBIOME:

  1. VAGINAL SELF-COLLECTION SWAB for the collection and stabilization of microbial DNA from vagina for microbiome analysis.

 

 

The new solution for home self-sampling, collection, shipping and easy processing in the laboratory

• Reduce waste of time for women that don’t want to take time off from work
• Absolute privacy
• Increased comfort
• Reduced anxiety

 

  1. REAL MICROBIOME VAGINAL DNA Kit has been designed for a fast and efficient purification of microbial DNA from vaginal samples.

More information 

Microbiome Fecal kit for DNA isolation from stool samples

REAL  MICROBIOME Fecal DNA kit has been designed for a fast and efficient purification of microbial DNA for MICROBIOME analysis from

1. Up to 200 mg fresh and frozen human or animal stool samples.

2. Stool homeganate from 0.50-1.0 gr stool and stabilized in 8 ml DANASTOOL Sample Collection MICROBIOME Kit.

More information

REAL Microbiome analysis of fecal samples

REAL has developed  a new kit for a fast and efficient purification of microbial DNA for the study of the microbiome from different faecal samples:

a) Up to 200 mg of human or animal feces, fresh or dried.

b) Stool samples (0-5-1.0 gr) homogenized and stabilized in 8ml REAL MICROBIOME Stool sample collection kit.

More information 

REAL Saliva DNA Swabs Sample Collection kit

Safe and rapid all in one method for the collection, stabilization and transport of saliva swabs samples.
Newsletter

Discover the New Real Fecal Microbiome Kit in Durviz

New Real Fecal Microbiome Kit

You can now buy the new Real Microbiome Fecal kit from the Durviz website. It is a new kit with which you can perform a rapid and efficient purification of microbial DNA for the study of it from different fecal samples.

A revolutionary faecal analysis system that you should know, especially if you perform this type of work in your laboratory.

 

New Real Microbiome kit: features

One of the main novelties of the Real Microbiome kit is that it includes an efficient lysis of the microorganisms present in fecal samples by a combination of heat and a chemical-mechanical break with specific “beads” developed for it. It should also be noted that the inhibitors of the samples are eliminated by precipitation through the use of a buffer especially developed for that.

The sample with which one works is passed, once cleaned of impurities, by the MicroSpin Column, the DNA is attached to the membrane and is washed and finally eluted.

Real Fecal Microbioma Kit

With all this, the main features of the new Real Microbiome Kit are:

  1. It is designed to obtain a fast and efficient purification of microbial DNA from different fecal samples.
  2. This optimally develops a lysis method with a combination of heat, chemical and mechanical lysis using specific “beads”. What allows a great homogenization of the sample that allows the isolation of the DNA of yeasts, fungi, Gram-negative and Gram-positive bacteria.
  3. It is a simple and fast procedure in which MicroSpin columns are used.
  4. One of its most revolutionary aspects is that it achieves a complete elimination of contaminants and inhibitors.
  5. Another outstanding aspect is that phenol / chloroform is not used in its operation; neither for extraction nor for precipitation with ethanol.

 

With all this, the new Real Kit is a product that can be used in many areas of work and study. For example, it has applications in the analysis of the microbiome, in the RFLP, in the analysis of mutations or even in the identification of pathogens.

 

Keep in mind that this kit works in optimal conditions for processing 200 mg of human feces. And that for the cases of animal samples it is recommended to reduce the amount of sample (80-100 mg). And it is that following these indications we will obtain better results.

In the case that we work with very dry stools, the manufacturers warn that the CTAB Extraction Buffer can absorb them, producing an insufficient amount of sample after the first centrifugation step.

In these cases, it is recommended to reduce the sample size (60-80 mg) and increase the volume of the CTAB Extraction Buffer.

 

The content of the Kit (in its standard size for 50 fecal samples) is as follows: a CTAB extraction buffer, an EC buffer, a binding buffer, a disinhibition buffer, a wash buffer, an elution buffer, 50 bead microtubes, proteinase K, 50 MicroSpin columns and 100 collection tubes. All of them are very well preserved at room temperature, except for the proteinase that should be at -20 degrees Celsius.

So, if you want your laboratory to have the latest in the extraction or purification of microbial DNA, do not hesitate and acquire the new Real Fecal Microbiome Kit. Your studies will have a smaller margin of error and will obtain much more data than you can process. Do not hesitate, get one of them already. You can get many more details on our website or through the phone.

circuling

The circulating DNA to diagnose possible genetic anomalies in fetuses

The circulating DNA to diagnose possible genetic anomalies in fetuses

The circulating DNA is that group of DNA molecules that circulates freely through the outside of the cells, by the blood, the urine, as well as in other body fluids. They are found in fragments of great length, that is to say, those that have from less from 500 bases to 21,000 base pairs.

It is also known as free DNA. The extraction of this DNA for its study in the laboratory is done through plasma or blood, since they are two easily manipulated and extractable liquids. To do so, it is important to have first quality materials that allow us a solid and accurate study of these cells of the organism, because through these reports we can diagnose different diseases.

Types of circulating DNA

According to the scientists, based on the origin of the circulating DNA sample, we can find three fundamental types:

  • Normal circulating DNA. It consists of degraded DNA fragments that they have been released into the bloodstream.
  • The circulating fetal DNA, which comes from mostly the placenta of the mothers. Actually it comes specifically from the fetus and it is a DNA that circulates freely in the bloodstream of the mother.
  • Tumor circulating DNA, which comes from cancer cells that have a Generally, of the tumor in which they are found. Your concentration is greater as the disease progresses.

Applications of circulating DNA

The circulating DNA is important because very information is extracted from its study valuable that will help us determine the state of health in which the person is from which the sample has been obtained. So, for example, in the case of fetuses we can help: diagnose possible genetic anomalies before birth; know the sex of the child before birth or without the need for any ultrasound (This is possible the analysis of specific sequences of the Y chromosome, which are performed following a presence / absence criterion); know the child’s RH to be able to rule out the hemolytic disease of the newborn (at this point you have to know that occasionally, the mother’s immune system produces antibodies during pregnancy they attack the red blood cells of their own fetus.

circulatingA fact that occurs, only, when the mother and the baby have different types of blood); determine diseases monogénicas (taking into account that with circulating DNA analysis that are performed at present, only diseases transmitted by the mother can be known and no for the mother); or study fetal aneuploidies (or, what is the same, alterations of the number of chromosomes that involve loss or gain of complete chromosomes).

And all this without the need to implement invasive techniques that endanger the life of the fetus or its mother. In this case, the test is done from the sixth week of pregnancy.

With the information obtained from the fetal circulating DNA analysis we can overtake many events and diseases, so it is vital importance its realization at present. It has even allowed to avoid treatments aggressive or complicated for pregnant mothers. Something common when there problems with the HR of the pregnant woman and the fetus. Situations in which the inclusion of analysis of the circulating DNA for the group pregnant Rh- has allowed the suppression of the immunoprophylaxis treatment when the fetus was also of the Rh- group.

In Durviz we have all the instruments you need to perform these tests. Do not you have more to visit our website and know the wide variety of products first quality we offer. A fact that has led us to be considered as one of the best business initiatives in the health sector worldwide.

real

REAL, extract and purify genomic DNA in blood

REAL, the best way to extract and purify genomic DNA in blood

Real is the name of the best method to extract and purify genomic DNA in blood in laboratories that “Durviz” company has launched to the market with the intention of helping the laboratories in performing all kinds of procedures in which it is necessary to manipulate DNA.

This process can be done, for example, using MicroSpin columns with silica membrane that selectively binds the DNA to perform the extraction and power handle it properly without any contamination. These products from REAL brand are manufactured and distributed by Durviz.

Realpure Spin Blood

Realpure Spin Blood is the name of a kit with which you can perform the extraction and the purification of genomic DNA from blood samples that the Durviz brand has been selling for years, obtaining excellent results among its customers.

realIn this case, the Kit uses a new lysis buffer specifically formulated for the extraction of DNA from blood samples. A procedure with which you get a high performance in the laboratory. That is, with which you get high quality DNA from blood, ready to use and quickly obtained. And all of it without using organic extractions or precipitations with alcohol, which supposes an advantage over other similar products that we can find in the market.

The DNA obtained by this procedure can be used directly in PCR, Southern, cloning and in any enzymatic reaction. The volume of elution obtained ranges between 50-200ul. The sample size is usually 300 ul total blood, plasma, serum and biological fluids.

For the correct functioning of this kit it is necessary to have, in addition, isopropanol, ethanol, 1.5 milliliter microtubes and a centrifuge.

Extraction and purification of DNA in blood

To carry out the extraction and purification in blood it is necessary to incubate the total blood in a chaotropic solution in the presence of proteinase K at 70 degrees centigrade.

In this way the optimal conditions will be created for the DNA to join a Silica fiber membrane when ethanol is added to this combination.

In order for the sample to be completely clean, contaminants are removed by two different washes. A procedure by which DNA is eluted with its own buffer for this procedure.

This system is appropriate especially when pursuing any of the following objectives:

  • Obtain extractions of genomic, viral and bacterial DNA.
  • Isolate and manipulate DNA from whole blood (human or source blood) animal, fresh or frozen).
  • Obtain DNA from blood treated with citrate, with EDTA, or with heparin.
  • DNA from serum, plasma, platelets, “Buffy coat”, fluids biological and so-called “dried blood spots”.

With all this, the company Durviz guarantees that the work is carried out in a more brief and with more guarantees.

 

New storage caps for Mini & MidiSystem

REAL has developed new screw caps compatible with the Mini and MiniSystem concentration devices.
These new caps allow to store in a simple and safe way the sediment after centrifugation, avoiding any odors or leaks.

REAL parasites poster available

Our new poster REAL Intestinal Parasites is now available!
Made in fixed stools using REAL MiniSystem, MidiSystem and Graham Test Safe & Clean.
Fixation media available: SAF, ECOSAF, MIF, TOTAL-FIX
Images owned by Dr. Guillermo Esteban. Professor of Parasitology. Faculty of Pharmacy, University of Valencia, Spain.
Request a poster for your laboratory or click here to download