COVID 19 detection

COVID19 detection solutions

At Durviz we have a wide range of products with which we can perform analyses that allow us to COVID 19  detection in our users. Solutions of very different types but always with excellent performance and high reliability in results.

For your knowledge, we’ve written this article that talks about the highlights.

 

COVID 19 detection solutions

Currently the need to test for the presence of COVID 19 coronavirus in people who request our services is growing by days. So it’s important to have solutions that enable us to respond effectively to each and every one of them, without being overworked or losing customers.

Companies in the sector have been able to react quickly to this need and have offered products in this regard. Different proposals that allow us to carry out mass analyzes in the best possible way, taking into account the circumstances in which they are usually carried out, and always providing reliable results.

Currently, at Durviz we offer the following products:

1- Real viral saliva. It is a complete kit that allows you to extract, manipulate and preserve saliva samples from each person in the best possible way. At room temperature or cold, so you can analyze it later in the lab. It is a multi-purpose product that is equally useful for testing DNA samples as infected RNA.

Real Saliva Viral. COVID 19 Detection

REAL Saliva Viral Collection Kit

2- Nasopharyngeal specimen collection and storage swabs. It is a product of the biocomma brand, which allows the sampling, facilitates the means of transport and conservation of virus (inactivator). This system avoids the risk of aerosol infection and is suitable for working with virus samples. It is a product valid for the collection and transport of different clinical samples such as influenza, avian influenza, HFMD and other viruses such as Mycoplasma, Ureaplasma, Chlamidia. And, of course, COVID coronavirus 19.

Virus transport and preservation medium . COVID 19 Detection

BIOCOMMA. – Virus transport and preservation medium

3- INTRON Biotechnology offers several products in this regard. On one hand, a series of kits with which DNA/RNA can be extracted from viruses. And, on the other, one called Real Time RT-PCR, which is just a kit for diagnostic COVID19. In his favor, it should be noted that the latter has been validated by the Carlos III Institute; and the National Center for Microbiology. This is always an extra guarantee.

Real Time RT-PCR. COVID 19 Detection

Real Time RT-PCR

4- MP Bio has One Step Strips called Immuno-Chromatography Cassette, which allow IgG/IgM to be detected from blood, serum and plasma samples. This means complete analyzes that allow us to detect anomalies. This product is for use only for in vitro diagnostic use by healthcare professionals.

Covid19 quick test for IgG/IgM detection.

Covid19 quick test for IgG/IgM detection

5- Alpha Laboratories has several products that make it easy for us to transport and send samples to the laboratory. They are as follows: SpeciSafe systems for the safe shipment of biological samples, contains absorbent foil according to UN3373. Features rigid packaging (secondary packaging); self-closing bags that allow safe shipment of biological samples with absorbent foil in compliance with UN3373 (secondary packaging); As well as an outer envelope or box (tertiary packaging) which, as stressed by UN3373, is suitable for sending biological samples by air or by land.

 

Remember that if you are interested or interested in acquiring any of them you only have to contact us and we will explain how to do it.

Real Saliva Viral. Saliva samples.

Real Saliva Viral: Brochure

Real viral saliva is a simple and practical kit that aims to bring speed and practicality to laboratory work. This novel product provides a safe and fast procedure for collecting, stabilizing and transporting saliva samples. Something fundamental especially now that large amounts of testing are being carried out by COVID 19. A procedure in which it has proven to be highly effective, allowing a large amount of sample collection to be made without any of them being harmed.

An all-in-one that allows us to work with 1 ml saliva samples at room temperature, allowing viral DNA/RNA to be stabilized without any problem. To do this, this product uses its own solution (contained in the kit itself) that inactivates viruses and prevents the degradation of nucleic acid in samples collected with our system. In this way, non-infectious samples are properly preserved for safe handling and sent.

The saliva samples we treat with this Real Saliva viral substance are kept at room temperature for a long period of time. Thus, in the case of DNA we have to talk about a period that extends over an entire year; and in the case of one-stage RNA that goes up to a month).

However, this is not the only advantage in or that of conservation. Saliva samples can also be frozen (-20/-80°C) for extended periods. A larger stage than other saliva testing kit offers.

 

Operation of a Real Saliva Viral

The way we can use this kit is very simple and is summarized in the following steps:

  1. Before removing the sample, people should be warned not to eat, drink, smoke or chew gum. Unless they haven’t done so 30 minutes before the sample was collected. Otherwise, it will be very difficult to get 1 ml of saliva. An extraction that can take several minutes in normal situations and that in the cases referred extends over time.
  2. The funnel must be inserted into the sample collection tube. It is advisable to rub the cheeks with the tongue and teeth for stimulation of saliva secretion.
  3. Collect saliva until 1 ml line level is reached.
  4. Remove and discard the funnel.
  5. Remove the cap from the saliva preservation solution and add all its contents to the collection tube.
  6. Close the sample collection tube with its green cap.
  7. Shake the tube vigorously for 15 seconds to mix saliva well with saliva preservation solution. At this point we must avoid unsolving saliva lumps.
  8. The saliva sample is already preserved and ready for storage, shipping and processing.
Real Saliva Viral

Kit Real Saliva Viral

Real Viral Saliva Apps

With all this Real viral saliva is much more effective than other similar products on the market, and they also offer the possibility to store saliva samples in the right way, for further analysis or treatment.

This kit presents all these application possibilities:

– It is a product specially designed for the collection, storage and transport of viral samples of 1 ml of saliva.

– Its substance inactivates microorganisms and viruses to enable safe and easy transport and handling.

Can be used to isolate human genomic DNA or viral DNA/RNA. In both cases you get excellent results.

Allows the sample to be transported at room temperature without adversely affecting it.

Facilitates the preservation of total DNA, including viral DNA, at room temperature (4º-25ºC) for an entire year, without any alteration.

Allows total RNA, including viral RNA, to be preserved at room temperature (4º-25ºC) for an entire month.

It is compatible with most of the DNA and RNA isolation methods on the market although the company recommends that the products and methods they manufacture be used as they are of very high quality standards and their greater efficiency has been proven in this regard.

Real Saliva Viral

Real Saliva Viral Collection Kit

So, if you are looking for a complete and simple kit, easy to handle and that allows you to collect and store saliva samples without any risk, this equipment of Real viral saliva is your best choice.

Real Saliva Viral

REAL Saliva Viral Collection Kit

Real viral is the name of a kit that provides a safe and fast procedure with which to collect, stabilize and transport saliva samples from 1 milliliter at room temperature. A procedure that stabilizes viral DNA/RNA in order to study it. This makes new and high-quality ways available to scientists to detect the presence of COVID 19 in people.

The main advantage of Real Viral is that it inactivates viruses and prevents the degradation of nucleic acid in samples collected with our system, resulting in non-infectious samples to be handled and sent safely.

Samples obtained by this procedure are kept at room temperature for one year for DNA and one month for RNA. In addition, they can be frozen (-20/ -80° C) for extended periods.

The kit contains the following:

  • 100 doses of 1 milliliter of the Real viral solution
  • 100 units of funnels
  • 100 saliva collection tubes

All of them are distributed at room temperature.

 

Features of the Real Saliva viral

The main features of this kit are:

  •  Used for the collection, storage and transport of viral samples of 1 ml of saliva.
  • Microorganisms and viruses are inactivated to make their transport and handling safe and easy.
  • This system can be used to isolate human genomic DNA or viral DNA/RNA, any of the procedures we need to perform.
  • Allows transport to be carried out at room temperature without affecting the sample.
  • Preserve total DNA, including viral DNA, at room temperature (4º-25ºC) for one year.
  • Preserve total RNA, including viral RNA, at room temperature (4º-25ºC)1 month.
  • It is a system compatible with most of the DNA and RNA isolation methods on the market.

For greater efficiency, from Durviz we recommend using our REAL Kits as a complement.

REAL SALIVA VIRAL

Real Saliva Viral

Use of Real Saliva Viral

To make proper use of this sample kit, follow the instructions below:

  1. Do not eat, drink, smoke or chew gum at least 30 minutes before collecting the sample. This way we make sure that the saliva is not contaminated. It should be remembered that to get 1 ml of saliva it can take several minutes.
  2. Insert the funnel into the sample collection tube. Then rub your cheeks with your tongue and teeth to stimulate saliva secretion.
  3. Collect saliva until it reaches the level of the 1 ml line of the tube. It is important to remove excess bubbles by hitting on a hard surface.  They should not be taken into account in the measurement.
  4. Remove and discard the funnel.
  5. Remove the cap from the saliva preservation solution and add all its contents in the collection tube with 1 ml of saliva.
  6. Close the sample collection tube with its green cap.
  7. Shake the tube vigorously for 15 seconds to mix saliva well with the saliva preservation solution. Prevent unsolving saliva lumps.
  8. The saliva sample is now preserved and ready for storage, shipping and processing.
Real Saliva Viral

Real Saliva Viral Collection Kit

With all this we will be able to do a proper research work and have results with a high degree of reliability.

So, if you want to benefit from the goodness of this product, remember that you only have to contact us and we will tell you what process you must follow for it.

New REAL Microbiome vaginal DNA kit

REAL has developed a complete system for the study of VAGINAL MICROBIOME:

  1. VAGINAL SELF-COLLECTION SWAB for the collection and stabilization of microbial DNA from vagina for microbiome analysis.

 

 

The new solution for home self-sampling, collection, shipping and easy processing in the laboratory

• Reduce waste of time for women that don’t want to take time off from work
• Absolute privacy
• Increased comfort
• Reduced anxiety

 

  1. REAL MICROBIOME VAGINAL DNA Kit has been designed for a fast and efficient purification of microbial DNA from vaginal samples.

More information 

Microbiome Fecal kit for DNA isolation from stool samples

REAL  MICROBIOME Fecal DNA kit has been designed for a fast and efficient purification of microbial DNA for MICROBIOME analysis from

1. Up to 200 mg fresh and frozen human or animal stool samples.

2. Stool homeganate from 0.50-1.0 gr stool and stabilized in 8 ml DANASTOOL Sample Collection MICROBIOME Kit.

More information

REAL Microbiome analysis of fecal samples

REAL has developed  a new kit for a fast and efficient purification of microbial DNA for the study of the microbiome from different faecal samples:

a) Up to 200 mg of human or animal feces, fresh or dried.

b) Stool samples (0-5-1.0 gr) homogenized and stabilized in 8ml REAL MICROBIOME Stool sample collection kit.

More information 

REAL Saliva DNA Swabs Sample Collection kit

Safe and rapid all in one method for the collection, stabilization and transport of saliva swabs samples.
Newsletter

Discover the New Real Fecal Microbiome Kit in Durviz

New Real Fecal Microbiome Kit

You can now buy the new Real Microbiome Fecal kit from the Durviz website. It is a new kit with which you can perform a rapid and efficient purification of microbial DNA for the study of it from different fecal samples.

A revolutionary faecal analysis system that you should know, especially if you perform this type of work in your laboratory.

 

New Real Microbiome kit: features

One of the main novelties of the Real Microbiome kit is that it includes an efficient lysis of the microorganisms present in fecal samples by a combination of heat and a chemical-mechanical break with specific “beads” developed for it. It should also be noted that the inhibitors of the samples are eliminated by precipitation through the use of a buffer especially developed for that.

The sample with which one works is passed, once cleaned of impurities, by the MicroSpin Column, the DNA is attached to the membrane and is washed and finally eluted.

Real Fecal Microbioma Kit

With all this, the main features of the new Real Microbiome Kit are:

  1. It is designed to obtain a fast and efficient purification of microbial DNA from different fecal samples.
  2. This optimally develops a lysis method with a combination of heat, chemical and mechanical lysis using specific “beads”. What allows a great homogenization of the sample that allows the isolation of the DNA of yeasts, fungi, Gram-negative and Gram-positive bacteria.
  3. It is a simple and fast procedure in which MicroSpin columns are used.
  4. One of its most revolutionary aspects is that it achieves a complete elimination of contaminants and inhibitors.
  5. Another outstanding aspect is that phenol / chloroform is not used in its operation; neither for extraction nor for precipitation with ethanol.

 

With all this, the new Real Kit is a product that can be used in many areas of work and study. For example, it has applications in the analysis of the microbiome, in the RFLP, in the analysis of mutations or even in the identification of pathogens.

 

Keep in mind that this kit works in optimal conditions for processing 200 mg of human feces. And that for the cases of animal samples it is recommended to reduce the amount of sample (80-100 mg). And it is that following these indications we will obtain better results.

In the case that we work with very dry stools, the manufacturers warn that the CTAB Extraction Buffer can absorb them, producing an insufficient amount of sample after the first centrifugation step.

In these cases, it is recommended to reduce the sample size (60-80 mg) and increase the volume of the CTAB Extraction Buffer.

 

The content of the Kit (in its standard size for 50 fecal samples) is as follows: a CTAB extraction buffer, an EC buffer, a binding buffer, a disinhibition buffer, a wash buffer, an elution buffer, 50 bead microtubes, proteinase K, 50 MicroSpin columns and 100 collection tubes. All of them are very well preserved at room temperature, except for the proteinase that should be at -20 degrees Celsius.

So, if you want your laboratory to have the latest in the extraction or purification of microbial DNA, do not hesitate and acquire the new Real Fecal Microbiome Kit. Your studies will have a smaller margin of error and will obtain much more data than you can process. Do not hesitate, get one of them already. You can get many more details on our website or through the phone.

circuling

The circulating DNA to diagnose possible genetic anomalies in fetuses

The circulating DNA to diagnose possible genetic anomalies in fetuses

The circulating DNA is that group of DNA molecules that circulates freely through the outside of the cells, by the blood, the urine, as well as in other body fluids. They are found in fragments of great length, that is to say, those that have from less from 500 bases to 21,000 base pairs.

It is also known as free DNA. The extraction of this DNA for its study in the laboratory is done through plasma or blood, since they are two easily manipulated and extractable liquids. To do so, it is important to have first quality materials that allow us a solid and accurate study of these cells of the organism, because through these reports we can diagnose different diseases.

Types of circulating DNA

According to the scientists, based on the origin of the circulating DNA sample, we can find three fundamental types:

  • Normal circulating DNA. It consists of degraded DNA fragments that they have been released into the bloodstream.
  • The circulating fetal DNA, which comes from mostly the placenta of the mothers. Actually it comes specifically from the fetus and it is a DNA that circulates freely in the bloodstream of the mother.
  • Tumor circulating DNA, which comes from cancer cells that have a Generally, of the tumor in which they are found. Your concentration is greater as the disease progresses.

Applications of circulating DNA

The circulating DNA is important because very information is extracted from its study valuable that will help us determine the state of health in which the person is from which the sample has been obtained. So, for example, in the case of fetuses we can help: diagnose possible genetic anomalies before birth; know the sex of the child before birth or without the need for any ultrasound (This is possible the analysis of specific sequences of the Y chromosome, which are performed following a presence / absence criterion); know the child’s RH to be able to rule out the hemolytic disease of the newborn (at this point you have to know that occasionally, the mother’s immune system produces antibodies during pregnancy they attack the red blood cells of their own fetus.

circulatingA fact that occurs, only, when the mother and the baby have different types of blood); determine diseases monogénicas (taking into account that with circulating DNA analysis that are performed at present, only diseases transmitted by the mother can be known and no for the mother); or study fetal aneuploidies (or, what is the same, alterations of the number of chromosomes that involve loss or gain of complete chromosomes).

And all this without the need to implement invasive techniques that endanger the life of the fetus or its mother. In this case, the test is done from the sixth week of pregnancy.

With the information obtained from the fetal circulating DNA analysis we can overtake many events and diseases, so it is vital importance its realization at present. It has even allowed to avoid treatments aggressive or complicated for pregnant mothers. Something common when there problems with the HR of the pregnant woman and the fetus. Situations in which the inclusion of analysis of the circulating DNA for the group pregnant Rh- has allowed the suppression of the immunoprophylaxis treatment when the fetus was also of the Rh- group.

In Durviz we have all the instruments you need to perform these tests. Do not you have more to visit our website and know the wide variety of products first quality we offer. A fact that has led us to be considered as one of the best business initiatives in the health sector worldwide.

real

REAL, extract and purify genomic DNA in blood

REAL, the best way to extract and purify genomic DNA in blood

Real is the name of the best method to extract and purify genomic DNA in blood in laboratories that “Durviz” company has launched to the market with the intention of helping the laboratories in performing all kinds of procedures in which it is necessary to manipulate DNA.

This process can be done, for example, using MicroSpin columns with silica membrane that selectively binds the DNA to perform the extraction and power handle it properly without any contamination. These products from REAL brand are manufactured and distributed by Durviz.

Realpure Spin Blood

Realpure Spin Blood is the name of a kit with which you can perform the extraction and the purification of genomic DNA from blood samples that the Durviz brand has been selling for years, obtaining excellent results among its customers.

realIn this case, the Kit uses a new lysis buffer specifically formulated for the extraction of DNA from blood samples. A procedure with which you get a high performance in the laboratory. That is, with which you get high quality DNA from blood, ready to use and quickly obtained. And all of it without using organic extractions or precipitations with alcohol, which supposes an advantage over other similar products that we can find in the market.

The DNA obtained by this procedure can be used directly in PCR, Southern, cloning and in any enzymatic reaction. The volume of elution obtained ranges between 50-200ul. The sample size is usually 300 ul total blood, plasma, serum and biological fluids.

For the correct functioning of this kit it is necessary to have, in addition, isopropanol, ethanol, 1.5 milliliter microtubes and a centrifuge.

Extraction and purification of DNA in blood

To carry out the extraction and purification in blood it is necessary to incubate the total blood in a chaotropic solution in the presence of proteinase K at 70 degrees centigrade.

In this way the optimal conditions will be created for the DNA to join a Silica fiber membrane when ethanol is added to this combination.

In order for the sample to be completely clean, contaminants are removed by two different washes. A procedure by which DNA is eluted with its own buffer for this procedure.

This system is appropriate especially when pursuing any of the following objectives:

  • Obtain extractions of genomic, viral and bacterial DNA.
  • Isolate and manipulate DNA from whole blood (human or source blood) animal, fresh or frozen).
  • Obtain DNA from blood treated with citrate, with EDTA, or with heparin.
  • DNA from serum, plasma, platelets, “Buffy coat”, fluids biological and so-called “dried blood spots”.

With all this, the company Durviz guarantees that the work is carried out in a more brief and with more guarantees.