REAL Crypto, Giardia, Entamoeba rapid test

Description

A rapid test for the qualitative detection of Cripto, Giardia and Entamoeba in human stool samples CE certified

Applications:

REAL Crypto, Giardia, Entamoeba Test Cassette (Feces) is a rapid chromatographic immunoassay for the qualitative detection of Crypto, Giardia and Entamoeba in human feces specimen.

Description:

Parasitary infections remain a very serious health problem worldwide. Crypto, Giardia, Entamoeba  are the most common protozoa known to be responsible for one of the main causes of severe diarrhoea in humans, particularly in immunodepressed people.
Epidemiological studies, in 1991, showed that infections with Giardia increased in the United States with a prevalence of around 6% on 178,000 samples. Generally, the disease passes through a short acute phase followed by a chronic phase. Infection by G. lamblia, in the acute phase, is the cause of watery diarrhoea with principally the elimination of trophozoites. The stools become normal again, during the chronic phase, with transient emissions of cysts.

The presence of the parasite on the wall of the duodenal epithelium is responsible for a malabsorption. The disappearance of villosities and their atrophy lead to problems with the digestive process at the level of the duodenum and the jejunum, followed by weight loss and dehydration. The majority of infections remain asymptomatic, however.

The diagnosis of G. lamblia is carried out under microscopy after flotation on zinc sulphate or by direct or indirect immunofluorescence, on non-concentrated samples displayed on a slide. More and more ELISA methods are also now available for the specific detection of cysts and/or trophozoïtes. Detection of this parasite in surface or distribution water can be undertaken by PCR type techniques.

Cryptosporidiosis is a diarrhoeal disease caused by microscopic parasites of the genus Cryptosporidium. Once an animal or person is infected, the parasite lives in the intestine and passes in the stool. The parasite is protected by an outer shell that allows it to survive outside the body for long periods of time and makes it very resistant to chlorine-based disinfectants. Both the disease and the parasite are commonly known as “Crypto.” The disease can spread through ingestion of contaminated water or through coughed fomites of an infected individual. It can spread by fecal-oral route like other gastrointestinal pathogens.

Entamoeba histolytica is an anaerobicparasiticamoebozoan, part of the genus Entamoeba. Predominantly infecting humans and other primates causing amoebiasis,E. histolyticais estimated to infect about 50 million people worldwide. Previously, it was thought that 10% of the world population was infected, but these figures predate the recognition that at least 90% of these infections were due to a second species, E. dispar. Mammals such as dogs and cats can become infected transiently, but are not thought to contribute significantly to transmission. E. histolytica, as its name suggests (histolytic= tissue destroying), ispathogenic; infection can be asymptomatic or can lead toamoebic dysenteryor amoebicliver abscess.

REAL has developed a rapid membrane test that can detect Crypto, Giardia, Entamoeba in non-concentrated faecal samples within 15 minutes.

Reference: RPPCGE25 (REAL Crypto, Giardia, Entamoeba Rapid Test; 25 units)

Protocol