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REAL Genomic DNA kit

SKU: RBME07-08-09-10-11-12-13-14-15-16 Categories: All Products, DNA isolation, Genomic DNA, Molecular Biology Tags: DNA, genomic, REAL
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Description

Different kits designed for an efficient and fast purification of highly pure genomic DNA from a wide variety of samples:

  • REAL GENOMIC DNA Cell kit: For purification of archive-quality DNA from cell cultures and cell suspensions.
  • REAL GENOMIC DNA Tissue kit: For purification of archive-quality DNA from tissues.
  • REAL GENOMIC DNA Mouse Tail kit: For purification of archive-quality DNA from mouse tails.
  • REAL GENOMIC DNA Bacteria kit: For purification of archive-quality DNA Gram-positive or Gram-negative bacteria.
  • REAL GENOMIC DNA Yeast kit: For purification of archive-quality DNA from yeast.

The process includes a cell lysis with an anionic detergent that solubilizes the necessary cell components, the contaminant RNA can be removed with a RNase treatment. The cell proteins are removed by precipitation, which allows to leave the genomic DNA in solution. Finally, the genomic DNA is isolated by a precipitation with isopropanol.

Features:

  • Reproducible,  fast and non-expensive method.
  • Convenient  and scalable purification procedure.
  • Safe method, as it removes completely the   need of using toxic reagents.
  • Allows to process different biological samples.
  • A high quality DNA is obtained, with an A260/280 of 1.7 – 1.9 ratio.

Applications:

DNA purified using this kit is highly stable and suited for use in a wide range of applications such as: 

  • DNA archiving.
  • PCR and quantitative real-time PCR.
  • SNP analysis.
  • Southern Blotting.
  • Sequencing.
REAL GENOMIC DNA KITS
Reference Description
RBME07 50 preps. Of 20 mg tissue
RBME08 200 preps. Of 20 mg tissue
RBME09 1650 preps. Of 20 mg tissue
RBME10 50 preps. of 3-5 x 10⁶ cells
RBME11 200 preps. of 3-5 x 10⁶ cells
RBME12 1650 preps. of 3-5 x 10⁶ cells
RBME13 For 200 preps. Of 0.5-1.0 cm mouse tail
RBME14 For 1650 preps. Of 0.5-1.0 cm mouse tail
RBME15 Volume culture processed 200 ml bacteria
RBME16 Volume culture processed 200 ml yeast

Additional information

Reference

RBME07, RBME08, RBME09, RBME10, RBME11, RBME12, RBME13, RBME14, RBME15, RBME16

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