Description
Different kits designed for an efficient and fast purification of highly pure genomic DNA from a wide variety of samples:
- REAL GENOMIC DNA Cell kit: For purification of archive-quality DNA from cell cultures and cell suspensions.
- REAL GENOMIC DNA Tissue kit: For purification of archive-quality DNA from tissues.
- REAL GENOMIC DNA Mouse Tail kit: For purification of archive-quality DNA from mouse tails.
- REAL GENOMIC DNA Bacteria kit: For purification of archive-quality DNA Gram-positive or Gram-negative bacteria.
- REAL GENOMIC DNA Yeast kit: For purification of archive-quality DNA from yeast.
The process includes a cell lysis with an anionic detergent that solubilizes the necessary cell components, the contaminant RNA can be removed with a RNase treatment. The cell proteins are removed by precipitation, which allows to leave the genomic DNA in solution. Finally, the genomic DNA is isolated by a precipitation with isopropanol.
Features:
- Reproducible, fast and non-expensive method.
- Convenient and scalable purification procedure.
- Safe method, as it removes completely the need of using toxic reagents.
- Allows to process different biological samples.
- A high quality DNA is obtained, with an A260/280 of 1.7 – 1.9 ratio.
Applications:
DNA purified using this kit is highly stable and suited for use in a wide range of applications such as:
- DNA archiving.
- PCR and quantitative real-time PCR.
- SNP analysis.
- Southern Blotting.
- Sequencing.
| Reference | Description | Documents |
| RBME07 | 50 preps. Of 20 mg tissue | Protocol |
| RBME08 | 200 preps. Of 20 mg tissue | Protocol |
| RBME09 | 1650 preps. Of 20 mg tissue | Protocol |
| RBME10 | 50 preps. of 3-5 x 10⁶ cells | Protocol |
| RBME11 | 200 preps. of 3-5 x 10⁶ cells | Protocol |
| RBME12 | 1650 preps. of 3-5 x 10⁶ cells | Protocol |
| RBME13 | For 200 preps. Of 0.5-1.0 cm mouse tail | Protocol |
| RBME14 | For 1650 preps. Of 0.5-1.0 cm mouse tail | Protocol |
| RBME15 | Volume culture processed 200 ml bacteria | Protocol |
| RBME16 | Volume culture processed 200 ml yeast | Protocol |
