REAL PLASMID MIDI/MAXIPREP kit

Description

REAL PLASMID MIDI/MAXIPREP kit

REAL PLASMID Midi/Maxiprep Kit offers a simple method for isolating plasmid DNA from 25-150 ml of recombinant E.coli cultures

This kit combines a modified alkaline lysis method with the convenience of anion-exchange columns to isolate high purity transfection grade plasmid DNA from bacterial cell lysates.

During the cell lysis step, both chromosomal and plasmid DNA are denatured. Potassium acetate is added to form a neutralized precipitate containing chromosomal DNA and other cellular components.

Plasmid DNA that remains in the solution, reverts to its native supercoiled structure, and then is loaded onto an equilibrated anion exchange column. The plasmid DNA binds to the anion exchange resin and then is eluted from the column with washing steps. Eluted DNA is precipitated and easily dissolved in TE buffer or nuclease free water.

The purified plasmids are suitable for use in the most demanding molecular biology applications, including transfection, in vitro transcription, automated or manual sequencing, cloning, hybridization and PCR.

It introduces TrueBLUE Lysis control reagent a color indicator wich provides visual identification of optimum buffer mixing. This prevent common handling errors that lead to inefficient cell lysis and incomplete precipitation of SDS, genomic DNA and cell debris. This makes ideal for use by researchers who have not much experience with plasmid preparation as well as experienced scientists who want to be assured of maximum product yield.

Features

• Spin columns with glass fiber membrane technology which promotes the selective DNA binding
• The customer chooses the size of the bacteria cultures which he wants to work with in a rango from 25-150 ml
• Plasmid DNA can be recovered from 25-150 ml of Luria Broth (LB) in about 45 mins
• Up to 500 ug yield of high copy plasmid DNA
• No risk of DNA pellet loss during precipitation
• Simple methos, fast and economical