• Facebook
  • Linkedin
  • Twitter
  • Youtube
  • Shop
  • My Account
  • Checkout
  • Basket
  • 0Shopping Cart
Real Laboratory
  • Home
    • About
  • Products
    • Sample collection & preservation
      • Saliva
      • Stool
      • Tissues/Cells
      • Microbiome
    • Molecular Biology
      • DNA purification
      • RNA purification
      • Microbiome
      • Reagents
    • Microbiology
      • Parasitology
  • Resources
    • Sample Collection
    • Molecular Biology
    • Microbiology
  • Distributors
  • News
  • Contact Us
  • Durviz SL
  • Search
  • Menu

Recent Posts

  • Detection of COVID19 in real time with LiliF COVID-19
  • Real viral saliva
  • SpeciSafe systems for the safe shipment of biological samples
  • Rapid coVID-19 and Influenza A+B antigen test
  • Viral DNA-RNA Purification Kit

Categories

  • Home
  • Microbiology

REALTOTAL RNA Spin Plants and Fungi

SKU: RBMER13 Categories: Molecular Biology, RNA isolation, Total RNA Tags: fungi, molecular biology, Plants, RBMER13, REAL, REALTOTAL, RNA, RNA isolation
  • Description
  • Reviews (0)

Description

REALTOTAL RNA Spin Plants and Fungi

 

This kit allows to obtain Total RNA from different cells and tissues of plants and fungi.

 

KIT DESCRIPTION:

 

The Kit is designed to obtain DNA-free total RNA from different cells and tissues of plants and fungi using silica membrane columns.

 

The samples are ground under liquid nitrogen followed by incubation in the lysis solution which immediately inactivates the RNases and creates suitable binding conditions for the RNA absorption on the silica membrane. Together with the lysis solution, a PVP (polyvinylpyrrolidone) solution is added, that acts binding contaminants such as polyssacharides and polyphenols wich may interfere or degrade the RNA. The contaminant DNA is removed by the application of a DNase I solution (supplied with the kit) directly on the column.

 

The salts, metabolites and cell components are removed by 2 washing steps. Finally, the RNA is eluted with nuclease-free water.

 

REALTOTAL RNA Spin Plants and Fungi contains 2 different lysis solutions, one based on guanidine thiocyanate, the most recommended due to its denaturing property. And other based on guanidine HCl as in some plants and fungi the presence of certain metabolites produces a solidification of the lysate, avoiding its processing, in such cases, the lysis solution with…

 

 

FEATURES:

 

–          Silica membrane column technology. Each column allows to bind 70ug of total RNA.

–          Includes filters to homogenize and reduce lysate viscosity, a PVP solution, and two different solutions and DNase I with a reaction buffer.

–          HQ RNA is obtained that can be used in several later applications (RT-PCR, Northern blotting, microarray…

–          Process can be done in less than 30 minutes.

–          0.5 – 5% of the RNA obtained is enough to do a RT-PCR.

–          Tested species: corn, tomato, vine, pine tree, olive tree, orange tree, Arabidopsis, pea, tobacco, wheat, barley, cucumber, lettuce, spinach, and Mucor rouxii and Phycomitrella patens fungi.

 

REFERENCES:

 

RBMER13: Extraction from 50 samples of fungi or plant cells.

 

PROTOCOL:

 

Click Here to download the protocol.

 

ORDERING:

 

To make an order, please contact to your distributor

Reviews

There are no reviews yet.

Be the first to review “REALTOTAL RNA Spin Plants and Fungi” Cancel reply

Your email address will not be published. Required fields are marked *

Related products

  • REAL Clean & Concentrator MicroSpin

    Read more Show Details
  • REAL FFPE DNA kit

    Read more
  • REAL SALIVA COLLECTION AND TRANSPORT DEVICE

    Read more Show Details
  • REALPURE SPIN BLOOD kit

    Read more

Schedule

Monday to Friday
8.30 – 17.00

Address

Leonardo Da Vinci, 10
Valencia Technology Park
46980 Paterna, Valencia, SPAIN

Real Laboratory SL

info@reallaboratory.com
Teléfono: +34 96 136 61 07
Fax: +34 96 136 61 68

Information

Buying Guide
General conditions
Standards and privacy ( LOPD )

© Copyright - Real is a Registered Trademark of Durviz S.L.U. All rights reserved. - 2016 - Web design by B2B activa.
  • Facebook
  • Linkedin
  • Twitter
  • Youtube
  • Home
  • Products
  • Resources
  • Distributors
  • News
  • Contact Us
  • Durviz SL
REALPLASMID SPIN MINIPREP KIT REALTOTAL RNA Spin Blood
Scroll to top