REAL, extract and purify genomic DNA in blood
REAL, the best way to extract and purify genomic DNA in blood
Real is the name of the best method to extract and purify genomic DNA in blood in laboratories that “Durviz” company has launched to the market with the intention of helping the laboratories in performing all kinds of procedures in which it is necessary to manipulate DNA.
This process can be done, for example, using MicroSpin columns with silica membrane that selectively binds the DNA to perform the extraction and power handle it properly without any contamination. These products from REAL brand are manufactured and distributed by Durviz.
Realpure Spin Blood
Realpure Spin Blood is the name of a kit with which you can perform the extraction and the purification of genomic DNA from blood samples that the Durviz brand has been selling for years, obtaining excellent results among its customers.
In this case, the Kit uses a new lysis buffer specifically formulated for the extraction of DNA from blood samples. A procedure with which you get a high performance in the laboratory. That is, with which you get high quality DNA from blood, ready to use and quickly obtained. And all of it without using organic extractions or precipitations with alcohol, which supposes an advantage over other similar products that we can find in the market.
The DNA obtained by this procedure can be used directly in PCR, Southern, cloning and in any enzymatic reaction. The volume of elution obtained ranges between 50-200ul. The sample size is usually 300 ul total blood, plasma, serum and biological fluids.
For the correct functioning of this kit it is necessary to have, in addition, isopropanol, ethanol, 1.5 milliliter microtubes and a centrifuge.
Extraction and purification of DNA in blood
To carry out the extraction and purification in blood it is necessary to incubate the total blood in a chaotropic solution in the presence of proteinase K at 70 degrees centigrade.
In this way the optimal conditions will be created for the DNA to join a Silica fiber membrane when ethanol is added to this combination.
In order for the sample to be completely clean, contaminants are removed by two different washes. A procedure by which DNA is eluted with its own buffer for this procedure.
This system is appropriate especially when pursuing any of the following objectives:
- Obtain extractions of genomic, viral and bacterial DNA.
- Isolate and manipulate DNA from whole blood (human or source blood) animal, fresh or frozen).
- Obtain DNA from blood treated with citrate, with EDTA, or with heparin.
- DNA from serum, plasma, platelets, “Buffy coat”, fluids biological and so-called “dried blood spots”.
With all this, the company Durviz guarantees that the work is carried out in a more brief and with more guarantees.