REAL Saliva RNA Kit

Description

Saliva, the most accessible and noninvasive biofluid of our body, harbors a wide spectrum of biological analytes informative for clinical diagnostic applications. Recently, human RNA obtained from saliva was shown to be a biomarker for several diseases.

REAL has developed a complete system that uses saliva as the sample source for expression studies.

REAL Saliva RNA Sample Collection Kit provides a safe and rapid all-in-one procedure for the collection, stabilization and transportation of1 ml saliva samples at ambient temperature that stabilizes RNA from the moment of collection for 1 month. The saliva collection tube is sent to the laboratory for RNA isolation and analysis using our REAL SALIVA RNA Kit.

REAL SALIVA RNA Kit has been designed for a fast and efficient purification of total RNA from preserved saliva samples.

The process includes a cell lysis with proteinase K followed by a precipitation of the proteins and part of genomic DNA. Later, by a precipitation with isopropanol, total RNA is obtained. Finally, for removal of genomic DNA contamination  is used an approach consisting of two sequential filtrations with different microSpin columns.

Features
• Buffer-based RNA isolation combined with gDNA removal with columns.
• Sample volume: 600 μl of preserved saliva sample.
• RNA is isolated without the use of harmful chemicals as phenol or chloroform.
• Total RNA:
• A260/A280 Ratio: >1.8
• Elution volume: 50 μl

Download Protocol