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Discover the New Real Fecal Microbiome Kit in Durviz

New Real Fecal Microbiome Kit

You can now buy the new Real Microbiome Fecal kit from the Durviz website. It is a new kit with which you can perform a rapid and efficient purification of microbial DNA for the study of it from different fecal samples.

A revolutionary faecal analysis system that you should know, especially if you perform this type of work in your laboratory.

 

New Real Microbiome kit: features

One of the main novelties of the Real Microbiome kit is that it includes an efficient lysis of the microorganisms present in fecal samples by a combination of heat and a chemical-mechanical break with specific “beads” developed for it. It should also be noted that the inhibitors of the samples are eliminated by precipitation through the use of a buffer especially developed for that.

The sample with which one works is passed, once cleaned of impurities, by the MicroSpin Column, the DNA is attached to the membrane and is washed and finally eluted.

Real Fecal Microbioma Kit

With all this, the main features of the new Real Microbiome Kit are:

  1. It is designed to obtain a fast and efficient purification of microbial DNA from different fecal samples.
  2. This optimally develops a lysis method with a combination of heat, chemical and mechanical lysis using specific “beads”. What allows a great homogenization of the sample that allows the isolation of the DNA of yeasts, fungi, Gram-negative and Gram-positive bacteria.
  3. It is a simple and fast procedure in which MicroSpin columns are used.
  4. One of its most revolutionary aspects is that it achieves a complete elimination of contaminants and inhibitors.
  5. Another outstanding aspect is that phenol / chloroform is not used in its operation; neither for extraction nor for precipitation with ethanol.

 

With all this, the new Real Kit is a product that can be used in many areas of work and study. For example, it has applications in the analysis of the microbiome, in the RFLP, in the analysis of mutations or even in the identification of pathogens.

 

Keep in mind that this kit works in optimal conditions for processing 200 mg of human feces. And that for the cases of animal samples it is recommended to reduce the amount of sample (80-100 mg). And it is that following these indications we will obtain better results.

In the case that we work with very dry stools, the manufacturers warn that the CTAB Extraction Buffer can absorb them, producing an insufficient amount of sample after the first centrifugation step.

In these cases, it is recommended to reduce the sample size (60-80 mg) and increase the volume of the CTAB Extraction Buffer.

 

The content of the Kit (in its standard size for 50 fecal samples) is as follows: a CTAB extraction buffer, an EC buffer, a binding buffer, a disinhibition buffer, a wash buffer, an elution buffer, 50 bead microtubes, proteinase K, 50 MicroSpin columns and 100 collection tubes. All of them are very well preserved at room temperature, except for the proteinase that should be at -20 degrees Celsius.

So, if you want your laboratory to have the latest in the extraction or purification of microbial DNA, do not hesitate and acquire the new Real Fecal Microbiome Kit. Your studies will have a smaller margin of error and will obtain much more data than you can process. Do not hesitate, get one of them already. You can get many more details on our website or through the phone.

system for stool sample conservation and concentration

Parasite concentrator system for stool sample

REAL Minisystem Faecal parasite concentrator system for stool sample conservation and concentration

Parasite concentrator systemMinisystem is a parasite concentrator system for stool sample conservation and concentration.

In July of 2010 Real Laboratory launched the New Mini System, after months of development, working close with our customers trying to solve the main problems and needs in the field. The result is a cleaner, smaller, more effective and ecological parasite collector.

Many of the human diseases are intestinal infections and diagnosis is based on the detection of helmith eggs and larvae or protozoa cysts in faecal samples. For this purpose an appropriate collection, transport and handling of those samples is critical.

  • Cleaner: The sample handling is minimum in all the process.
  • Smaller: The space saving is a real deal in the labs nowadays.DSC03969
  • More effective: The double filtration allows to get better results with less volume of sample.
  • Ecological: Due to its size, the process is done with less reagents, contributing to minimize the environmental effects of those.

Minisystem has a new cap design with a longer spoon makes the system even cleaner as it protects the user from touching the sample and at the same time its longer spoon makes the collection of the sample easier. The first filter holds gross matter of the sample and the second clarifies and allows parasites or bacterias pass, its pore size. Therefore, it results in a sediment for analysis with fewer impurities.

Only with Durviz, you can get this system with glass beads to help homogenization of the sample.

Our customers can choose among several fixation methods: SAF, ECOSAF, MIF and TOTAL-FIX BUFFER, to concentrate the parasite structures.

Minisystem accessories availables

DSC03985

 

Complementarily, the Easy pick system is designed for collection of sediment after centrifugation, it includes a pasteur pipette and a special pierceable cap that fit on the sediment cone.

This system allows the user to store the sediment avoiding bad odours and at the same time provides the user an easy and confortable system for collecting the sediment for its placement on the slide.

Possibility to purchase only the cap, only the pipette or the entire system.

 

REAL MiniSystem use. Sample collection:

  1. Defecate in a clean dry recipient.
  2. Avoid contaminating of faeces with urine or water.
  3. Choose an adequate amount of faeces and put it into the preservative tube using the cap-spoon. The quantity of sample to pick up should not exceed the capacity of the tube. Not to open the inferior cap of the tube in any moment.
  4. Close well the tube and send it to the laboratory for processing.

Parasite concentrator system with Minisystem

Minisystem protocolo ENG

  1. Sample collection (explained above)
  2. Sample homogeneization: Shake vigorously the collection tube with the sample for 30 seconds.
  3. Double filtration and concentration: Exchange the bottom cap for the sedimentation cone and put it in the machine for centrifugation.
  4. Sediment recovery with Easy Pick System (optional).
  5. Sediment storage or disposal.

We recommended storage in high quality refrigeration systems to not reduce sediment properties.

MiniSystem is a closed system for stool sample conservation and concentration of REAL Laboratory, trademark property of Durviz. Request for samples so you can see its benefits.