Description
Designed for a fast and efficient purification of microbial DNA for MICROBIOME analysis from up to 200 mg fresh and frozen human or animal stool samples.
In this procedure, the microorganisms are efficiently lysed by a combination of heat, chemical and mechanical disruption with specialized beads. Inhibitos are eliminated by precipitation using a propietary clean up buffer. The sample is then applied to a microspin column and the DNA that is bound to the column undergoes a single wash step before elution.
Specifications:
• Designed for a fast and easy purification microbial DNA from different types of stool samples.
• Silica-membrane technology with MiniSpin columns.
Optimized lysis method-Combination of heat, chemical and mechanical lysis via bead-based homogenization enables isolation of DNA from yeast, fungi, Gram-negative and Gram-positive bacteria.
• Eliminates inhibitory substances, including lipids, polysaccharides and heme.
• Typical yield: Approx. 5-60 µg depends on sample type.
• Preparation Time: 35 min.
• Elution volume: 200 µl.
• No phenol/cloroform extraction or ethanol precipitation is necessary.
Applications:
• Microbiome analysis
• PCR applications.
• RFLP analysis.
• Patogehn typing.
• Mutation analysis
| Reference | Description |
| RBMEGS16 | For 50 preps |
