REAL Clean & Concentration Kit

Description

This kit provides a rapid method for purification and concentration of high-quality DNA from PCR or enzymatic reactions with an extremely small elution volume of only 10 ml using specials MicroSpin columns.

Applications:

• CR products clean-up, efficient desalting of DNA with the removal of DNA polymerases, primers and free dNTPs.
• DNA clean-up from Enzymatic Reactions, including: Desphosphorylation, Restriction enzyme digestion, Ligation, Primed synthesis, End labeling and Nick translation.
• Isotope and Dye Removal, efficiently removes unicorporated fluorescent ( i.e., AMCA, FITC, BIO, DIG, Cy3, Cy5, FAM, etc) and radiolabeled dNTP derivats from DNA following in vitro labeling reactions.

Features:

• The microspin columns are designed to allow elution in very small volumes (as little as 10 ml) delivering highly concentrated DNA in high yields.
• DNA Size Limits: from 70 pb to 23 kb.
• DNA Recovery: tipically, up to 5 mg total DNA per column can be eluted into as little as 10 ml.
• The protocol is done is 2 minutes.
• Fast procedure and easy handling.
• Eluted DNA is well suited for use in DNA ligation, sequencing, labelling, PCR, etc.

References:

Ref.RBMCS04     50 purifications

Ref.RBMCS05     250 purifications

Ref.RBMCS09     1000 purifications

Click here for the protocol.