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REAL Clean & Concentration Kit

SKU: RBMCS04-05-09 Categories: All Products, DNA Clean Up, Molecular Biology, PLANT Tags: ADN, DNA, dna fragments, dna purification, molecular biology, PCR reactions, post-reaction, RBMCS04, RBMCS05, REAL
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Description

This kit provides a rapid method for purification and concentration of high-quality DNA from PCR or enzymatic reactions with an extremely small elution volume of only 10 ml using specials MicroSpin columns.

Applications:

  • CR products clean-up, efficient desalting of DNA with the removal of DNA polymerases, primers and free dNTPs.
  • DNA clean-up from Enzymatic Reactions, including: Desphosphorylation, Restriction enzyme digestion, Ligation, Primed synthesis, End labeling and Nick translation.
  • Isotope and Dye Removal, efficiently removes unicorporated fluorescent ( i.e., AMCA, FITC, BIO, DIG, Cy3, Cy5, FAM, etc) and radiolabeled dNTP derivats from DNA following in vitro labeling reactions.

 

Features:

  • The microspin columns are designed to allow elution in very small volumes (as little as 10 ml) delivering highly concentrated DNA in high yields.
  • DNA Size Limits: from 70 pb to 23 kb.
  • DNA Recovery: tipically, up to 5 mg total DNA per column can be eluted into as little as 10 ml.
  • The protocol is done is 2 minutes.
  • Fast procedure and easy handling.
  • Eluted DNA is well suited for use in DNA ligation, sequencing, labelling, PCR, etc.

 

References:

Ref.RBMCS04     50 purifications

Ref.RBMCS05     250 purifications

Ref.RBMCS09     1000 purifications

 

Click here for the protocol.

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