• Facebook
  • Linkedin
  • Twitter
  • Youtube
  • Shop
  • My Account
  • Checkout
  • Basket
  • 0Shopping Cart
Real Laboratory
  • Home
    • About
  • Products
    • Sample collection & preservation
      • Saliva
      • Stool
      • Tissues/Cells
      • Microbiome
    • Molecular Biology
      • DNA purification
      • RNA purification
      • Microbiome
      • Reagents
    • Microbiology
      • Parasitology
  • Resources
    • Sample Collection
    • Molecular Biology
    • Microbiology
  • Distributors
  • News
  • Contact Us
  • Durviz SL
  • Search
  • Menu

Recent Posts

  • Detection of COVID19 in real time with LiliF COVID-19
  • Real viral saliva
  • SpeciSafe systems for the safe shipment of biological samples
  • Rapid coVID-19 and Influenza A+B antigen test
  • Viral DNA-RNA Purification Kit

Categories

  • Home
  • Microbiology

REAL Microbiome oral DNA

Categories: Microbiomics, Molecular Biology
  • Description
  • Reviews (0)

Description

The microorganisms found in the human oral cavity have been referred to as the oral microflora, oral microbiota, or more recently as the oral microbiome.
Human oral cavity harbors the second most abundant microbiota after the gastrointestinal tract.
The oral microbiome is one of the most diverse of any human-associated microbial community . The oral microbiome is a causative factor in conditions such as dental caries , periodontal disease , and halitosis , and has also been implicated as a reservoir for infection at other body sites  and in the pathogenesis of non-oral diseases, such as inflammatory bowel disease .
REAL has complete system for the study of ORAL MICROBIOME:

  1. REAL SALIVA Sample Collection MICROBIOME Kit is An all-in-one collection kit for the collection and stabilization of microbial DNA from saliva.
    It contains a non-lytic buffer that preserves microbial community at least 1 year. The stabilization is instant and the buffer prevents bacterial growth, so the sample provides a representative snapshot of the microbial community that remains unchanged from collection to processing.
  2. REAL MICROBIOME SALIVA DNA Kit has been designed for a fast and efficient purification of microbial DNA from saliva samples.

Beginning with a bead-beating protocol, cells are lysed through a combination of mechanical force, heat and detergent, vortexed using horizontal adapter for the Vortex Genie 2 Vortex or using others common disruption devices.

Appropriate DNA binding conditions to the Microbial DNA Columns are achieved by addition of large amounts of chaotropic salts (Binding Buffer) to the lysate. Contaminants are removed by two efficient washing steps. Afterwards, The resulting DNA is recovered in a DNA-free Tris buffer to use for subsequent reactions.

 

Microbial composition of saliva sample preserved at room temperature is unchanged after  two months with REAL SALIVA Sample Collection MICROBIOME Kit . Saliva samples were taken using our system and stored at room temperature. They were sampled at the indicated time points and processed with the REAL MICROBIOME SALIVA DNA Kit. The extrated DNA was the subjected to microbial composition profiling via 16S rRNA gene targeted sequencing. Samples had a constant microbial composition.Fig.1

 


Fig.1 Saliva Samples with REAL SALIVA Sample Collection MICROBIOME Kit -Species

 

REAL SALIVA Sample Collection MICROBIOME Kit
• All-in-one devices for optimal self-collection
• Standardize sample collection
• Stabilize microbial DNA at ambient temperature at least 1 year
• Provide a snapshot of the saliva microbiome.
• Suitable for NGS downstream applications

References:
Ref. RBMSALMC100: REAL SALIVA SAMPLE COLLECTION MICROBIOME KIT 100 UNITS
Ref. RBMSALMC1000: REAL SALIVA SAMPLE COLLECTION MICROBIOME KIT 1000 UNITS
Ref. RBMSALMC100CE: REAL SALIVA SAMPLE COLLECTION MICROBIOME KIT 100 UNITS CE Mark
Ref. RBMSALMC1000CE: REAL SALIVA SAMPLE COLLECTION MICROBIOME KIT 1000 UNITS CE Mark

 

REAL MICROBIOME SALIVA DNA Kit

• Designed for rapid purification of highly pure microbial DNA for microbiome analysis.
• Silica-membrane technology with MiniSpin columns.
• Bead Microtubes for efficient lysis included in combination liquid Proteinase K.
• Sample material: saliva / preserved saliva samples.
• Typical yield: Approx. 2-20 µg depends on patient.
• Preparation Time: 35 min.
• Elution volume: 100 µl.

References:

RBMEGS19: REAL MICROBIOME SALIVA DNA KIT 50 PREPS
RBMEGS19CE: REAL MICROBIOME SALIVA DNA KIT 50 PREPS CE Mark

 

Protocol

Reviews

There are no reviews yet.

Be the first to review “REAL Microbiome oral DNA” Cancel reply

Your email address will not be published. Required fields are marked *

Related products

  • REAL PLANT DNA kit

    Read more
  • REALTOTAL RNA Spin Blood

    Read more Show Details
  • REALStock

    Read more
  • REALPURE Plants and Fungi

    Read more Show Details

Schedule

Monday to Friday
8.30 – 17.00

Address

Leonardo Da Vinci, 10
Valencia Technology Park
46980 Paterna, Valencia, SPAIN

Real Laboratory SL

info@reallaboratory.com
Teléfono: +34 96 136 61 07
Fax: +34 96 136 61 68

Information

Buying Guide
General conditions
Standards and privacy ( LOPD )

© Copyright - Real is a Registered Trademark of Durviz S.L.U. All rights reserved. - 2016 - Web design by B2B activa.
  • Facebook
  • Linkedin
  • Twitter
  • Youtube
  • Home
  • Products
  • Resources
  • Distributors
  • News
  • Contact Us
  • Durviz SL
REAL Stool Sample Collection kit REAL Saliva Collection RNA
Scroll to top