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REAL FFPE DNA Kit

SKU: RBMEGS12 Categories: All Products, DNA isolation, Genomic DNA, Molecular Biology
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Description

his kit is optimized for a fast method to isolate DNA from formalin-fixed, paraffin-embedded (FFPE) tissue specimen.

The procedure omits the use of flammable and malodorous xylene or d-limonene commonly used for desparaffinization, a proprietary buffer formulation  DEPARAFFINIZATION SOLUTION is used for the complete dissolution of the wax to release the tissue.

Procedure:

  1. Remove paraffin: paraffin is dissolved and removal in the DEPARAFFINIZATION SOLUTION.
  2. Lyse: sample is lysed under denaturing conditions with    proteinase K.
  3. Heat: incubation at 90ºC reverses formalin crosslinking.
  4. Bind: DNA binds  to the membrane and contaminants flow through.
  5. Wash: residual contaminants are washed away.
  6. Elute; concentrated DNA is elute from the membrane.

Features:

  • Silica membrane technology with specials MicroSpin columns.
  • Very easy paraffin removal..
  • Safe method avoids xylene and other toxic.
  • Complete removal  of contaminants and inhibitors for reliable downstream applications.
  • Low elution volume: 15-30 μl.
  • The  quality of DNA is suitable for the following applications as  quantitative PCR or Next generation sequencing (NGS).

Applications:

  • Rapid isolation of DNA from formalin-fixed, paraffin-embedded samples.
  • Isolation of DNA from fresh and archived FFPE samples.
  • Isolation of DNA from specimen of object slides.
  • NGS, STR analysis.

REAL.- FFPE DNA KIT
Reference Description
RBMEGS12 For 50 preps

Additional information

Reference

RBMEGS12

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