REAL FFPE DNA kit

Description

REAL FFPE DNA kit

his kit is optimized for a fast method to isolate DNA from formalin-fixed, paraffin-embedded (FFPE) tissue specimen.

The procedure omits the use of flammable and malodorous xylene or d-limonene commonly used for desparaffinization, a proprietary buffer formulation  DEPARAFFINIZATION SOLUTION is used for the complete dissolution of the wax to release the tissue.

Procedure:

1. Remove paraffin: paraffin is dissolved and removal in the DEPARAFFINIZATION SOLUTION.
2. Lyse: sample is lysed under denaturing conditions with    proteinase K.
3. Heat: incubation at 90ºC reverses formalin crosslinking.
4. Bind: DNA binds  to the membrane and contaminants flow through.
5. Wash: residual contaminants are washed away.
6. Elute; concentrated DNA is elute from the membrane.

Features:

• Silica membrane technology with specials MicroSpin columns.
• Very easy paraffin removal..
• Safe method avoids xylene and other toxic.
• Complete removal  of contaminants and inhibitors for reliable downstream applications.
• Low elution volume: 15-30 μl.
• The  quality of DNA is suitable for the following applications as  quantitative PCR or Next generation sequencing (NGS).

Applications:

• Rapid isolation of DNA from formalin-fixed, paraffin-embedded samples.
• Isolation of DNA from fresh and archived FFPE samples.
• Isolation of DNA from specimen of object slides.
• NGS, STR analysis.