REAL Swabs DNA Kit

Description

We presented this kit for an efficient and fast  purification of highly pure genomic DNA from different saliva samples including:

1. Buccal swabs
2. Preserved buccal cells collected with our REAL Swabs Sample Collection kit

This process includes a cell lysis with an anionic detergent that solubilizes the necessary cell components, proteinase K and RNase. The cell proteins are removed by precipitation, which allows to leave the genomic DNA in solution. Finally, the genomic DNA is isolated by a precipitation with isopropanol.

Features:

• DNA from saliva is equivalent to DNA from blood for downstream applications.
• Improve patient care and compliance with painless, non-invasive sample collection and decreases costs.
• Reproducible,  fast and non-expensive method.
• Safe method, as it removes completely the   need of using toxic reagents.

Applications:

DNA purified using REAL Swabs DNA Kit is highly stable and suited for use in a wide range of applications such as:

• DNA archiving.
• PCR and quantitative real-time PCR.
• SNP analysis.
• Southern Blotting.
• Next Generation Sequencing.

Protocol