This kit provides a method for an efficient and fast total RNA from tissues and cells using MiniSpin columns.
REAL TISSUE/CELLS RNA kit integrates a gDNA Removal column. This Mini spin column removes quickly and efficiently most genomic DNA without the need of DNase digestion.
In the first stwp cells and tissues are lysed without the need of beta mercapthoethanol. The chaotropic salt includes in the lysis buffer immediatelly inactivates Rnases. The lysate is added to the gDNA Removal Column to clarify the Lysate and to remove contaminating gDNA. After addition of the binding solution to the flow through, the RNA is bound to the RNA column. Afterwards, two washing steps remove salts, metabolites and macromolecular cellular components. High quality RNA is eluted with RNase free H2O.
- Fast procedure delivering high quality total RNA in minutes
- Convenient handling lysate, clearing and gDNA removal with on column in one step
- Sample material:<1 x 10 7 cultured cells; 25 mg animal/human tissue
- No phenol/chloroform extraction, no CsCl, no gradients, no LiCl or ethanol precipitation
- RNA isolation from cultures cells and animal tissues
- RNA is ready for downstream applications such as RT PCR, Northern Blotting, Primer Extension, mRNA Selection, cDNA Synthesis, RNase Protection ELUTION Assay.
|REAL TISSSUE/CELLS RNA KIT